Purification Propensity for Proteins
from Bacillus halodurans
The demand
for proteins with special purposes increases significantly, for example,
special proteins are in good need in development of sensitive, specific and
reliable differential diagnostic assays. To meet such huge demand, proteins of
interest can be expressed in either prokaryotic or eukaryotic cells, like
Escherichia coli, to produce recombinant proteins. However, this is not an easy
task and is often costly. Purification of recombinant proteins from
#plantbiomass currently accounts for almost 80% of production cost. A series of
difficulties may be encountered in #purification. For instance, purified
proteins from a host may accumulate in low titers and may be mixed with
infection or form #proteinaggregates. Therefore a purification scheme usually
includes many steps, such as affinity chromatography, precipitation, protecting
of recombinant proteins from degradation with stabilizer, centrifugation and so
on.
The Coming Age of Future Medicine:
Next Frontier
The era of
modern medicine is driven by recent advances in #bioanalytical and
bioinformatics technologies and the novel insights into #humanbiology that are
emerging through the application of these technologies. Physics and its
discoveries have been at the forefront of medical diagnosis and treatment since
the discovery of X-rays in 1895. Since then, #biologicalresearches have been
renovating from a descriptive or phenomenological to a quantitative and
predictive discipline by employing the physics covering possible degrees of
freedom leading to changes in the way biological systems are understood.
Rutherford’s elucidation of atomic structure had amalgamated physics, chemistry
and biology to better understands nature.
By
Chandrasekharan
III B.
Sc.,
Department
of biochemistry
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